how to grow your own bioluminescent algae

Step 1:Gather the Materials

A number of marine enthusiasts already grow phytoplankton at home for use in feeding various species of marine life. The method we’ll use is rather similar.

To start, you need,

  1. A clear growing container (old 2l soda bottle)
  2. Sea Salt
  3. A grow light and timer
  4. Micro Algae Grow
  5. Optional: Small, motorized air pump
  6. A Starter Culture

Sea Salt: No, not from your pantry you gourmet fiend, you can get this at most pet or aquarium stores.
http://www.petsmart.com/product/index.jsp?productId=2754897

Grow Light: you can pick up a plant fluorescent and rack from walmart for ~$10…. Make sure you have a light timer.

Micro Algae grow: our most crucial ingredient. (besides the actual algae) There are a number of nutrient formulas people have experimented with, and truthfully, I’ve only had mixed results with this one. Experiment with what works best. You can get it here:
https://3kserver7.com/~frank/secure/agora.cgi

Air Pump: this is used to both circulate the nutrients as well as keep vital CO2 in the water. The algae need this to grow.

A Starter Culture: These can be obtained from a few places online. I’ve used sunnyside sea farms.
http://seafarms.com/html/products.html

INTERNATIONAL SHIPPING (Canada, Mexico, Europe and Great Britain)
All shipments outside of the USA are FED EX International Priority.
Only (3) 50 ml Bags and (30) 3 ml vials are available for Overseas shipping. (40$-50$)

Step 2:Preparation and Mixing

Sanitation is necessary so your batch doesn’t crash. After you REALLY wash out the soda bottle make sure there is absolutely no residue left. Some people say swirl some diluted bleach around in the bottle. Others say to stick it in the microwave after it’s completely dry (won’t melt or deform if it’s dry… wet is another story). Choose your preference.

Additionally, sanitize the tubing if you’re using an air pump, and anything else you’re using to prepare this batch.

Mix up a batch of salt water. I’ve used purified water, however some people swear by their tap water as it adds in some minerals needed for the algae to grow. I’m sure the composition of minerals in tap water varies significantly from region to region, so I’ll let you make that decision. For help, you can check out some of the discussions on the topic at http://reefcentral.com/forums/index.php?s=8a3a4cfe49aebd49cdce4f3ae79c8c08

Regardless, mix the salt to a 1.019 specific gravity (sg) concentration. Directions on how to do this are on the back of the package… you’ll need a hydrometer if you’ve never done it before.

Add in ~ 1 ml of the micro algae grow. In this case, less is more. The solution you received the culture in should already have enough nutrients to support sizable growth. If you don’t want to mess with making your own solution (not necessarily a bad idea) many places that sell starter cultures will also sell culture solution.

Let both the solution and culture bag sit in the same area out of the sun for an hour or two. This is simply to let them reach room temperature. A sudden change in temp during transfer could shock the culture enough to significantly harm it. If your room temp is in the 70s, you should be okay. If my memory is correct, they grow ideally if the water temp is in the mid to upper 60s.

Finally, transfer the algae into your bottles. If you are using the air pump, be sure to cut a hole in the top of the bottle cap. When I say air pump, keep in mind I really only mean a few bubbles every 10-20 seconds or so. We don’t want to disturb these dinoflagellites too much – just ressuply their CO2. I’ve even heard of people using another tank of yeast (which emit CO2) as their air supply. Attached is a picture of a grower’s setup. (Your bottles won’t be green though)

Step 3:Growth

These dinoflagellates need a constant cycle of light and darkness for optimal growth. Put your grow light and bottles in a dark place (closet) where you can strictly control how much light they get. Set the timer so the grow light is on a cycle of 12 hours on, 12 hours off. Don’t be worried if your starter culture doesn’t emit light right after you receive it. They will only bioluminesce in their night cycle, so plan the light cycles accordingly for when you want to see it.

Monitor your cultures for any sudden changes in color, and give them a gentle shake every day or so or all the sediment will collect to the bottom. If you have a successful culture, you will eventually need to ‘split’ the batch. Mix up another batch of saltwater/nutrients, and halve your culture between the new bottles.

Remember, these cool creatures will only brightly flash when disturbed. They have a ‘recharge’ time so to speak between disturbances for optimal performance.

If you’re looking for something which will constantly glow, you might want to check out bioluminescent bacterium instead. You can get some from Carolina Biological supply. Culturing this is a rather different process, but you can find some guides on the net. One bioluminescent strain is Vibrio fischeri. See here: http://www.carolina.com/product/living+organisms/prokaryotes/bacteria/vibrio+anguillarum%2C+living%2C+tube.do?keyword=Vibrio+fischeri&sortby=bestMatches&page=1
The pictures on this page are mostly from this site: http://www.biology.pl/bakterie_sw/bac_pict_en.html

About these ads

~ door ilseleysen op 02/12/2009.

Geef een reactie

Vul je gegevens in of klik op een icoon om in te loggen.

WordPress.com logo

Je reageert onder je WordPress.com account. Log uit / Bijwerken )

Twitter-afbeelding

Je reageert onder je Twitter account. Log uit / Bijwerken )

Facebook foto

Je reageert onder je Facebook account. Log uit / Bijwerken )

Google+ photo

Je reageert onder je Google+ account. Log uit / Bijwerken )

Verbinden met %s

 
Volg

Ontvang elk nieuw bericht direct in je inbox.

%d bloggers like this: